Friday, October 12, 2012

Mechanisms of enhanced osteoblast gene expression in the presence of hydroxyapatite coated iron oxide magnetic nanoparticles

Nhiem Tran, Douglas Hall and Thomas J Webster



Hydroxyapatite (HA) coated iron oxide (Fe 3 O 4 ) magnetic nanoparticles have been shown to enhance osteoblast (bone forming cells) proliferation and osteoblast differentiation into calcium depositing cells (through increased secretion of alkaline phosphatase, collagen and calcium deposition) compared to control samples without nanoparticles. Such nanoparticles are, thus, very promising for numerous orthopedic applications including magnetically directed osteoporosis treatment. The objective of the current study was to elucidate the mechanisms of the aforementioned improved osteoblast responses in the presence of HA coated Fe 3 O 4 nanoparticles. Results demonstrated large amounts of fibronectin (a protein known to increase osteoblast functions) adsorption on HA coated Fe 3 O 4 nanoparticles. Specifically, fibronectin adsorption almost doubled when HA coated Fe 3 O 4 nanoparticle concentrations increased ...



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