Design of a VLP-nanovehicle for CYP450 enzymatic activity delivery

Background: The intracellular delivery of enzymes for therapeutic use has a promising future for the treatment of several diseases such as genetic disorders and cancer. Virus-like particles offer an interesting platform for enzymatic delivery to targeted cells because of their great cargo capacity and the enhancement of the biocatalyst stability towards several factors important in the practical application of these nanoparticles. Results: We have designed a nano-bioreactor based on the encapsulation of a cytochrome P450 (CYP) inside the capsid derived from the bacteriophage P22. An enhanced peroxigenase, CYPBM3, was selected as a model enzyme because of its potential in enzyme prodrug therapy. A total of 109 enzymes per capsid were encapsulated with a 70 % retention of activity for cytochromes with the correct incorporation of the heme cofactor. Upon encapsulation, the stability of the enzyme towards protease degradation and acidic pH was increased. Cytochrome P450 activity was delivered into Human cervix carcinoma cells via transfecting P22-CYP nanoparticles with lipofectamine. Conclusion: This work provides a clear demonstration of the potential of biocatalytic virus-like particles as medical relevant enzymatic delivery vehicles for clinical applications.
Lorena Sánchez-Sánchez
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Synthesis and Luminescent Properties of REVO4–REPO4 (RE = Y, Eu, Gd, Er, Tm, or Yb) Heteronanostructures: A Promising Class of Phosphors for Excitation from NIR to VUV

The Journal of Physical Chemistry C

DOI: 10.1021/acs.jpcc.5b08249

Paulo C. de Sousa Filho, Thierry Gacoin, Jean-Pierre Boilot, Richard I. Walton and Osvaldo A. Serra
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Heat Treatment Effect on Crystalline Structure and Photoelectrochemical Properties of Anodic TiO2 Nanotube Arrays Formed in Ethylene Glycol and Glycerol Based Electrolytes

The Journal of Physical Chemistry C

DOI: 10.1021/acs.jpcc.5b08403

Magdalena Jarosz, Karolina Syrek, Joanna Kapusta-Kołodziej, Justyna Mech, Kamilla Małek, Katarzyna Hnida, Tomasz Łojewski, Marian Jaskuła and Grzegorz D. Sulka
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Degradation Mechanisms of Organic Light-Emitting Diodes Based on Thermally Activated Delayed Fluorescence Molecules

The Journal of Physical Chemistry C

DOI: 10.1021/acs.jpcc.5b07084

Atula S. D. Sandanayaka, Toshinori Matsushima and Chihaya Adachi
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Gate-Tunable Electron Transport Phenomena in Al–Ge⟨111⟩–Al Nanowire Heterostructures

Nano Letters

DOI: 10.1021/acs.nanolett.5b03169

Florian M. Brunbauer, Emmerich Bertagnolli and Alois Lugstein
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Mussel-Inspired Multidentate Block Copolymer to Stabilize Ultrasmall Superparamagnetic Fe3O4 for Magnetic Resonance Imaging Contrast Enhancement and Excellent Colloidal Stability

Chemistry of Materials

DOI: 10.1021/acs.chemmater.5b03028

Puzhen Li, Pascale Chevallier, Parsarm Ramrup, Depannita Biswas, Dajana Vuckovich, Marc-André Fortin and Jung Kwon Oh
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Hexagonal Ceria Located at the Interface of Anatase/Rutile TiO2 Superstructure Optimized for High Activity under Combined UV and Visible-Light Irradiation

The Journal of Physical Chemistry C

DOI: 10.1021/acs.jpcc.5b05652

Ranjana Verma, S. K. Samdarshi and Jay Singh
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Mechanical and Electrical Anisotropy of Few-Layer Black Phosphorus

ACS Nano

DOI: 10.1021/acsnano.5b05151

Jin Tao, Wanfu Shen, Sen Wu, Lu Liu, Zhihong Feng, Chao Wang, Chunguang Hu, Pei Yao, Hao Zhang, Wei Pang, Xuexin Duan, Jing Liu, Chongwu Zhou and Daihua Zhang
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Cellulose Nanocrystal Microcapsules as Tunable Cages for Nano- and Microparticles

ACS Nano

DOI: 10.1021/acsnano.5b03905

Chunhong Ye, Sidney T. Malak, Kesong Hu, Weibin Wu and Vladimir V. Tsukruk
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IR-Driven Photocatalytic Water Splitting with WO2–NaxWO3 Hybrid Conductor Material

Nano Letters

DOI: 10.1021/acs.nanolett.5b01581

Guanwei Cui, Wen Wang, Mingyue Ma, Junfeng Xie, Xifeng Shi, Ning Deng, Jianping Xin and Bo Tang
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Oxidative Etching and Metal Overgrowth of Gold Nanorods within Mesoporous Silica Shells

Chemistry of Materials

DOI: 10.1021/acs.chemmater.5b03749

Tian-Song Deng, Jessi E. S. van der Hoeven, Anil O. Yalcin, Henny W. Zandbergen, Marijn A. van Huis and Alfons van Blaaderen
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Stable Pervanadyl Cation Encapsulated in Silica: Frivolous Vanadium in the H4PVMo11O40 Keggin

The Journal of Physical Chemistry C

DOI: 10.1021/acs.jpcc.5b08039

Wallace O. Parker and Fabrizio Cavani
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Experimental and Theoretical Analysis of Hydrogen Bonding in Two-Dimensional Chiral 4′,4′′′′-(1,4-Phenylene)bis(2,2′:6′,2″-terpyridine) Self-Assembled Nanoarchitecture

The Journal of Physical Chemistry C

DOI: 10.1021/acs.jpcc.5b07231

Manuela Mura and Fabien Silly
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Influence of Poly(N-vinylcarbazole) as a Photoanode Component in Enhancing the Performance of a Dye-Sensitized Solar Cell

The Journal of Physical Chemistry C

DOI: 10.1021/acs.jpcc.5b06582

Alagar Ramar, Ramiah Saraswathi, Muniyandi Rajkumar and Shen-Ming Chen
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Structure of Quaternary Ammonium Ionic Liquids at Interfaces: Effects of Cation Tail Modification with Isoelectronic Groups

The Journal of Physical Chemistry C

DOI: 10.1021/acs.jpcc.5b06460

Shobha Sharma and Hemant K. Kashyap
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Characterizing the Greater-Than-Bulk Melting Behavior of Ga–Al Nanoalloys

The Journal of Physical Chemistry C

DOI: 10.1021/acs.jpcc.5b04930

Udbhav Ojha and Nicola Gaston
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Core/Shell Face-Centered Tetragonal FePd/Pd Nanoparticles as an Efficient Non-Pt Catalyst for the Oxygen Reduction Reaction

ACS Nano

DOI: 10.1021/acsnano.5b04361

Guangming Jiang, Huiyuan Zhu, Xu Zhang, Bo Shen, Liheng Wu, Sen Zhang, Gang Lu, Zhongbiao Wu and Shouheng Sun
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Effect of Auger Recombination on Lasing in Heterostructured Quantum Dots with Engineered Core/Shell Interfaces

Nano Letters

DOI: 10.1021/acs.nanolett.5b02595

Young-Shin Park, Wan Ki Bae, Thomas Baker, Jaehoon Lim and Victor I. Klimov
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