Glucose availability determines silver nanoparticles toxicity in HepG2

Background: The increasing body of evidence suggest that nanomaterials toxicity is associated with generation of oxidative stress. In this paper we investigated the role of respiration in silver nanoparticles (AgNPs) generated oxidative stress and toxicity. Since cancer cells rely on glucose as the main source of energy supply, glucose availability might be an important determinant of NPs toxicity. Methods: AgNPs of 20 nm nominal diameter were used as a model NPs. HepG2 cells were cultured in the media with high (25 mM) or low (5.5 mM) glucose content and treated with 20 nm AgNPs. AgNPs-induced toxicity was tested by neutral red assay. Generation of H 2 O 2 in mitochondria was evaluated by use of mitochondria specific protein indicator HyPer-Mito. Expression of a 77 oxidative stress related genes was assessed by qPCR. The activity of antioxidant enzymes was estimated colorimetrically by dedicated methods in cell homogenates. Results: AgNPs-induced dose-dependent generation of H 2 O 2 and toxicity was observed. Toxicity of AgNPs towards cells maintained in the low glucose medium was significantly lower than the toxicity towards cells growing in the high glucose concentration. Scarceness of glucose supply resulted in upregulation of the endogenous antioxidant defence mechanisms that in turn alleviated AgNPs dependent ROS generation and toxicity. Conclusion: Glucose availability can modify toxicity of AgNPs via elevation of antioxidant defence triggered by oxidative stress resulted from enhanced oxidative phosphorylation in mitochondria and associated generation of ROS. Presented results strengthen the idea of strong linkage between NPs toxicity and intracellular respiration and possibly other mitochondria dependent processes.
Mariusz Zuberek
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Nano Letters

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The Journal of Physical Chemistry C

DOI: 10.1021/acs.jpcc.5b07207

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ACS Nano

DOI: 10.1021/acsnano.5b05660

Yangyang Liu, Su-Young Moon, Joseph T. Hupp and Omar K. Farha
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ACS Nano

DOI: 10.1021/acsnano.5b04027

Qinghua Ji, Xiaoqiang An, Huijuan Liu, Lin Guo and Jiuhui Qu
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The Journal of Physical Chemistry C

DOI: 10.1021/acs.jpcc.5b07960

C. Medina-Plaza, M. L. Rodriguez-Mendez, P. Sutter, X. Tong and E. Sutter
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The Journal of Physical Chemistry C

DOI: 10.1021/acs.jpcc.5b08341

Stefan Knoppe, Hannu Häkkinen and Thierry Verbiest
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The Journal of Physical Chemistry C

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The Journal of Physical Chemistry C

DOI: 10.1021/acs.jpcc.5b09256

Yi Zhang, Yongfeng Liu, Xin Zhang, You Li, Mingxia Gao and Hongge Pan
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Nano Letters

DOI: 10.1021/acs.nanolett.5b03451

John P. Mathew, Raj Patel, Abhinandan Borah, Carina B. Maliakkal, T. S. Abhilash and Mandar M. Deshmukh
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Self-Assembling VO2 Nanonet with High Switching Performance at Wafer-Scale

Chemistry of Materials

DOI: 10.1021/acs.chemmater.5b03314

Jiasong Zhang, Haibo Jin, Zhuo Chen, Maosheng Cao, Pengwan Chen, Yankun Dou, Yongjie Zhao and Jingbo Li
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Biomimetic Nanotubes Based on Cyclodextrins for Ion-Channel Applications

Nano Letters

DOI: 10.1021/acs.nanolett.5b03938

Hajar Mamad-Hemouch, Hassen Ramoul, Mohammad Abou Taha, Laurent Bacri, Cécile Huin, Cédric Przybylski, Abdelghani Oukhaled, Bénédicte Thiébot, Gilles Patriarche, Nathalie Jarroux and Juan Pelta
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Comment on “Breakdown of Interference Rules in Azulene, a Nonalternant Hydrocarbon”

Nano Letters

DOI: 10.1021/acs.nanolett.5b03468

Robert Stadler
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ACS Nano

DOI: 10.1021/acsnano.5b05609

Qiang Liu, Miao Zhang, Liang Huang, Yingru Li, Ji Chen, Chun Li and Gaoquan Shi
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Optical Second Harmonic Generation in Plasmonic Nanostructures: From Fundamental Principles to Advanced Applications

ACS Nano

DOI: 10.1021/acsnano.5b04373

Jérémy Butet, Pierre-François Brevet and Olivier J. F. Martin
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