Background: Magnetic nanoparticles functionalized antibodies are used for in-vitro assays on bio-markers. This work demonstrates the synthesis of high-quality magnetic nanoparticles coated with antibodies against carcinoembryonic antigen (CEA). Various characterizations, such as particle size, particle suspension, bio-activity and the stability of bio-magnetic nanoparticles suspended in liquid, are studied. The properties for the assay of CEA molecules in serum are also studied. The assay method used is so-called immunomagnetic reduction. Results: The results show that the effects of common materials in serum that interfere with detected signals are not significant. The low-detection limit is 0.21?ng/ml, which is well below the clinical threshold of 2.5?ng/ml. Conclusions: The dynamic range for the assay of CEA molecules in serum is 500?ng/ml. By assaying serum CEA molecules from 24 normal controls and 30 colorectal-cancer patients, the threshold for the serum-CEA concentration to diagnose colorectal cancer is 4.05?ng/ml, which results in a clinical sensitivity of 0.90 and specificity of 0.87.
Che-Chuan Yang
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Che-Chuan Yang
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