Background: Cell culture conditions can greatly influence the results of nanoparticle (NP) uptake assays. In this study, 10?nm gold nanoparticles (AuNPs) and RAW 264.7 macrophages were used as a model system, while instrumental neutron activation analysis (NAA) was used as the elemental analysis technique to determine AuNP levels produced by the various culturing conditions. Static plate-based and insert-based culture conditions were compared with a dynamic suspension culture to evaluate the conditions? effect on the rate and extent of AuNP uptake. Results: The results indicate that a dynamic culturing condition allows for the greatest NP uptake (approximately 3-5 times over the adherent conditions), whereas the plate-based assays have the initial highest rate of NP incorporation. Conclusions: These data highlight the importance of judiciously choosing the assay conditions prior to evaluating NP uptake.
Simona Bancos
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Simona Bancos
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